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. 2015 Mar 9;5:8866. doi: 10.1038/srep08866

Figure 1. Specific miRNAs are Important for the Heat Stress Response.

Figure 1

(a) Synchronized L4 wild type animals were exposed to 35°C for 4 hrs. Total RNA was isolated from this sample in addition to aged-matched control animals cultivated at 20°C. Both RNA samples were sequenced on the Illumina platform and analyzed with miRDeep2. (b) Deletion mutants of differentially expressed miRNAs affect survival at 32°C. Each mutant strain was incubated at 32°C for 15, 20 and 25 hrs and recovered for 24 hrs at 20°C. We used adult animals for these assays, and not L4 larvae as were used in the RNA sequencing, as heat stress assays using L4 animals are inconsistent in our hands. Heat stress assays were performed in triplicate. Data are presented as means ± SEM and statistical significance was assessed by 2way ANOVA followed by Tukey's multiple comparison test. n > 110, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.