Table 1. Largest miRNA Expression Changes in Heat Stress.

miRNA	Fold change	Homology
Most upregulated at 35°C
mir-83-3p	4.9
mir-239b-5p	3.7	mir-12
mir-49-3p	3.3
mir-249-3p	2.2
mir-1820-5p	2.1
Most downregulated at 35°C
mir-77-5p	−7.1	mir-4647
mir-81-5p	−6.6
mir-86-3p	−5.6
mir-51-3p	−4.9
mir-52-3p	−4.7
mir-237-3p	−3.5	mir-125
mir-58-5p	−3.2
mir-80-5p	−3.5	mir-143
mir-54-5p	−3.2
mir-84-3p	−2.5	let-7
mir-74-5p	−2.2	mir-185
mir-799	−2.1
mir-229-3p	−2.1
mir-71-3p	−1.9

Small RNA high-throughput sequencing analysis of control (20°C) and heat stress (35°C) samples revealed 19 miRNAs with >1.9-fold change, based on uniquely mapped, normalized read counts and regression analysis. miRNAs that are members of miRNA families conserved in other phyla are indicated.