Figure 3.

Sex-specific DNA methylation differences in the developing fetal brain. A total of 8059 (1.99% of the 408,594 autosomal and X-linked probes assessed) were identified as significantly differentially methylated between males and females in the fetal brain, with most differences occurring on the X Chromosome as expected. However, a number of autosomal loci also show significant differences between males and females, as illustrated by the four probes shown in A. Of note, DNA methylation at a number of (B) autosomal and (C) X Chromosome probes showed significant interactions between sex and developmental stage, indicating differential trajectories of DNA methylation in the developing male and female brain.