Assembly validation using 4D bin-mapped ESTs. Green bars represent 4D short arm binned EST k-mers present in the 454 short and long arm assembly (all contigs), respectively. Black bars represent 4D long arm binned EST k-mers present in the 454 long and short arm assembly (all contigs), respectively. Legend of Bins: Bin1: 4DS2-0.82–1.00, Bin2: 4DS3-0.67–0.82, Bin3: 4DS1-0.53–0.67, Bin4: 4DS1-0.53, Bin5: 4DL9-0.31, Bin6: 4DL9-0.31–0.56, Bin7: 4DL13-0.56–0.71, Bin8: 4DL12-0.71–1.00.