Fig. 1.

Histogram of relative DAPI fluorescence (flow karyotype). Histogram obtained after flow cytometric analysis of mitotic metaphase chromosomes of double ditelosomic line 4D of bread wheat cv. Chinese Spring. Peaks corresponding to telosomes 4DS and 4DL are well discriminated, which facilitated their flow sorting. Sorted chromosome arms were identified after FISH with probes for Afa repeat (yellow-green), which results in characteristic banding pattern (inset). X-axis: relative DAPI fluorescence intensity; Y-axis: number of particles.