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. 2015 Jan 21;14(2):243–252. doi: 10.4161/15384101.2014.977112

Figure 3.

Figure 3.

STAT3 directly regulated miR-146a expression in HCC. (A) A ChIP assay was performed 24 h after STAT3 decoy ODN (Dec) or scramble ODN (Scr) treatment to evaluate the recruitment of STAT3 on miR-146a promoter (miR146a-pro). (B) The level of phosphorylated STAT3 (p-STAT3) (Tyr705) in IL-6–stimulated (400 U/mL) HepG2 cells was examined by western blotting (upper), and a qPCR assay was used to detect the expression of miR-146a in HepG2 cells (lower). (C) After IL-6 stimulation, a ChIP-PCR assay was performed using an anti–p-STAT3705 antibody or rabbit IgG as a control. (D) The luciferase activity of the miR-146a promoter (miR146a-pro) in HepG2 cells was measured using a dual-GloTM Luciferase assay system. The ratio of firefly to Renilla luciferase activity with pGL3-TK-Luciferase transfection was set as 1. Data are representative of 3 independent experiments, and statistical significance was determined as **P < 0.01 and *P < 0.05 compared to control.