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. 2015 Jan 2;14(1):146–156. doi: 10.4161/15384101.2014.967106

Figure 4.

Figure 4.

For figure legend, see page 166.Figure 4 (See previous page). The WW and TAD domains are important for TAZ induced mammary tumor formation. (A) Ectopic expression of TAZ-S89A, TAZ-S311A, TAZ-2SA (S89A, S311A), TAZ-4SA in human non-transformed breast epithelial MCF10A cells as revealed by immunoblot. β-Actin was used as the loading control. (B) Quantifications of mammosphere formation of vector or TAZ-S89A, TAZ-S311A, TAZ-2SA (S89A, S311A) and TAZ-4SA transduced MCF10A cells. Bars denote standard errors (n = 6). (C) Flow cytometry analysis of CD44high/CD24low population in vector or TAZ-S89A, TAZ-S311A, TAZ-2SA (S89A, S311A) and TAZ-4SA transduced MCF10A cells. Percentage of CD44high/CD24low subpopulation is indicated. (D) Ectopic expression of TAZ-4SA, TAZ-4SAΔWW, TAZ-4SAΔTAD or TAZ-4SAΔPDZ in human non-transformed breast epithelial MCF10A cells as revealed by immunoblot. β-Actin was used as the loading control. (E) Flow cytometry analysis of CD44high/CD24low population in vector, TAZ-4SAΔWW, TAZ-4SAΔTAD and TAZ-4SAΔPDZ transduced MCF10A cells. Percentage of CD44high/CD24low subpopulation is indicated. (F) Quantifications of mammosphere formation of vector, TAZ-4SAΔWW, TAZ-4SAΔTAD, TAZ-4SAΔPDZ and TAZ-4SA transduced MCF10A cells. Bars denote standard errors (n = 6). (G) TAZ-4SA and TAZ-4SAΔPDZ-transduced MCF10A cells induce mammary tumor formation when injected into the mammary fat pad of NOD/SCID mice (n = 6). Representative images of induced mammary tumors are shown (Right panel).