Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Mar 1;142(5):846–857. doi: 10.1242/dev.116897

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015. Published by The Company of Biologists Ltd

PMC Copyright notice

Fig. 5. — Characterization of lineage decisions by single CPCs during in vitro differentiation. (A) The lineage decision for each single CPC during in vitro differentiation was determined through profiling the gene expression in each individual progeny of that CPC. (B) Representative lineage trees of single CPCs (black dots) based on time-lapse video microscopy data. Note the varying behaviors of each CPC. (C) Identity of lineage progenies from single eGFP⁺ CPCs that have been isolated from day 5 (D5) and 7 (D7) of in vitro mESC differentiation. (D) Same as in C, except that Nkx2-5–eGFP mouse embryo-derived eGFP⁺ cells at E7.5 and E8.5 of development were studied. (E) Summary of the frequency that each single Nkx2-5–eGFP⁺ cell became a CM, SMC/FB or EDC during in vitro differentiation. (F) Immunofluorescence staining of CPC progenies for specific cell-lineage markers. Scale bars: 100 µm. Data represent the results from two independent experiments.