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. 2015 Mar 1;142(5):910–920. doi: 10.1242/dev.116996

Fig. 2.

Fig. 2.

Effect of pancRNA knockdown on the expression of the counterpart gene during early development. (A) 5′-regions of mouse Il17d, Mospd3 and Tbc1d22a. Vertical lines mark the locations of CpG dinucleotides. Thick horizontal lines denote the regions analyzed by bisulfite sequencing. Primer positions are numbered relative to the TSS of each gene. (B) qPCR analysis of pancRNA and mRNA at the Il17d, Mospd3 and Tbc1d22a loci in MII oocytes and in fertilized 1-cell, 2-cell and 4-cell embryos. Error bars indicate s.e.m. (C) DNA methylation levels of the promoter regions of Il17d, Mospd3 and Tbc1d22a in MII oocytes, sperm and fertilized 1-cell and 2-cell embryos. Filled and open circles indicate methylated and unmethylated cytosines, respectively. *P<0.05; **P<0.01; ***P<0.001.