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. 2015 Mar 1;142(5):910–920. doi: 10.1242/dev.116996

Fig. 3.

Fig. 3.

Effect of knockdown of pancRNAs on partnered gene expression and on DNA methylation. (A) Expression levels of the indicated pancRNAs and their genes measured by qPCR in siRNA-injected 2-cell and 4-cell embryos. Il17d expression was not detectable (N.D.) in 2-cell embryos. (B) DNA methylation levels of the corresponding promoters in siRNA-injected embryos. The regions analyzed are displayed in Fig. 1A. (C) Effect of pancRNA knockdown on blastocyst formation. Asterisks indicate significant differences compared with si Control samples. The numbers of embryos used for injection of si Control, si pancIl17d, si pancMospd3 and si pancTbc1d22a siRNAs were 261, 251, 78 and 70, respectively. *P<0.05; **P<0.01; ***P<0.001. Error bars indicate s.e.m.