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. 2012 Feb 5;7(4):295–301. doi: 10.3969/j.issn.1673-5374.2012.04.010

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Copyright: © Neural Regeneration Research

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Axonal regeneration in retinal ganglion cells after optic nerve crush (immunofluorescence staining; fluorescein isothiocyanate-labeling; scale bar: 100 μm).

Representative photomicrographs show the crush site (asterisk) and regenerating axons with growth associated protein-43 immunofluorescence (arrowheads) on day 15 after injury.

Numerous growth associated protein-43 positive axons are seen passing through the crush site and they grew beyond the distal crush site in the EPO group (A). In contrast, very few axons traversed the crush site in the PBS group (B). In addition, the sham-surgery group (C) showed no staining. PBS: Phosphate buffered saline; EPO: erythropoietin.