Figure 2.

Alpha-synuclein protein expression in rat substantia nigra (immunofluorescence double-labeling staining, × 200).

Thioflavin S was used as a chromogenic marker of amyloid substance. Red fluorescence represents α-synuclein staining, and green represents thioflavin S staining. In the vehicle-treated (model) group, co-labeling of α-synuclein and thioflavin S was evident. In the Anchanling group, the co-labeling was reduced compared with the model group. Only weak red and green fluorescence was observed in the control group.