Table 1.
Citrus putative PH5-like gene sequences and their corresponding primers for quantitative real-time PCR.
| Gene name | Sequence ID | ORF amino acid length | Mol. Wt (KDa) | pI | Primer name | Sequence (5′–3′) | Amplicon size (bp) | |
|---|---|---|---|---|---|---|---|---|
| Forward primer | Reverse primer | |||||||
| CsPH1 | Cs5g04360.1 | 955 | 105.098 | 6.24 | PH1 | GCTCTCACAGATTTGGTGGT | CACAGCCTCCAAAACTTCCT | 165 |
| CsPH2 | Cs6g20570.1 | 957 | 105.299 | 6.16 | PH2 | GAGGCAGTGTTGAAGGAAGC | GGTTCCACATAAACCCCAAA | 190 |
| CsPH3 | Ciclev10013498m | 936 | 103.237 | 7.68 | PH3 | ACGAAGCAGTTACGGAGAAC | AGTGATTCGACGTGCCCTTT | 108 |
| CsPH4 | Cs7g07300.1 | 955 | 105.024 | 6.73 | PH4 | GTCTTCAACCACCCGAAACA | AGCTTCACCACCGATTCAAC | 154 |
| CsPH5 | orange1.1g002208m | 954 | 104.949 | 6.66 | PH5 | ACCCTTCATGGGCTTCAAC | GCTTCACCACTGACTCGACA | 163 |
| CsPH6 | Cs4g01370.1 | 950 | 104.428 | 6.29 | PH6 | CAGGGTTGAAAACCAGGATG | TCCATTGCTGTCGATGTAGG | 151 |
| CsPH7 | Ciclev10024879m | 860 | 94.177 | 5.33 | PH7 | ACAGGCTGTCTCAGCAAGGT | GATCTTTCTCCACCCCCTTC | 165 |
| CsPH8 | Cs1g16150.1 | 953 | 104.923 | 5.89 | PH8 | CCGTGAAGGAATTGATTTGG | CCATGACAATGGATTCCACA | 190 |
| Actin | XM_006464503 | – | – | – | actin | CCGACCGTATGAGCAAGGAAA | TTCCTGTGGACAATGGATGGA | 200 |