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. 2015 Mar 6;466(Pt 3):489–498. doi: 10.1042/BJ20141349

Figure 1. The UCH family of DUBs cleave Ub-rhodamine-110-Gly but are inactive towards Ub dimers.

Figure 1

(A) Domain maps of each of the UCH DUBs and the broadly active positive control USP2. (B) A 1 μg amount of each DUB was fractionated by SDS/PAGE and visualized by Coomassie Blue staining. (C) DUB activity assessed against the artificial substrate Ub-rhodamine-110-Gly. (D) Structure of linear Ub–Ub, PDBL 2W9N [23]. DUBs were incubated with linear Ub–Ub dimers and the reaction products were fractionated by SDS/PAGE and visualized by Coomassie Blue staining. Asterisks (*) denote the DUB in each reaction. DUB assays were carried out for 1 h at 37°C.