Table 1. Steady-state kinetic parameters for UCH family members.

	Enzyme	Km (μM)	Vmax (pmol·min−1)	kcat (s−1)	kcat/Km (M−1·s−1)
Ub-SUMO2
	UCHL1	5.22±0.49	0.03152	0.0000136	2.60
	UCHL3	4.94±0.70	0.2	0.000192	3.89×101
Ub-4xS2ΔN11
	UCHL3	1.37±0.14	8.93	0.00789	5.76×103
	BAP1	0.72±0.09	5.84	0.0104	1.45×104
Ub-Ube2W
	UCHL3	1.58±0.14	7.13	0.00630	3.99×103
	UCHL5	6.30±0.93	0.9	0.10	1.59×104
	BAP1	1.34±0.20	6.65	0.0119	8.86×103
Ub-(Δ 1–15) SUMO2
	UCHL3	0.29±0.05	1.27	0.00112	3.89×103
Ub-K11-SUMO2
	UCHL3	0.51±0.09	1.23	0.00107	2.08×103