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. 2015 Feb 1;14(3):323–332. doi: 10.4161/15384101.2014.980643

Figure 4.

Figure 4.

Lithium induces a TCF3/TCF4 exchange at the MYC 3′ WRE. (A) qPCR analysis of MYC expression in cDNAs prepared from HCT116 and FHs 74 Int cells that were untreated or treated with 25 mM LiCl for 6 hours. MYC levels are normalized to GAPDH. (B) Western blot analysis of MYC protein levels in control and LiCl-treated cells. (C) qPCR analysis of DNA fragments precipitated using anti-TCF4 antibodies in ChIP assays conducted in cells treated with LiCl as indicated. Shown are levels of TCF4 binding at the MYC 3′ WRE. (D) As in C except anti-β-catenin antibodies were used in the ChIP assays. (E) As in C except anti-TCF3 antibodies were used in the ChIP assays. All experiments were repeated at least 3 times and error bars are ± SEM (**P < 0.01, ***P < 0.001).