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. 2015 Feb 6;14(3):437–448. doi: 10.4161/15384101.2014.972901

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Figure 6. — The impact of Chk2 phosphorylation on BRCA1 stability and ubiquitination. (A) Comparison of phosphorylated Brca1 level in Brca1^+/+ and Brca1^S971A/S971A MEFs upon γIR (10 Gy). One representative experiment from 4 independent experiments. Brca1 is detected by an antibody recognizing mouse Brca1 (a gift of Richard Baer). (B) Quantification of phospho-Brca1 levels normalized by tubulin levels. Average of 4 independent experiments. Trend line represents polynomial fit of data. P = 0.001 using pairwise t-test. (C) Comparison of in vivo ubiquitination of FLAG-tagged BRCA1(wt) and BRCA1(S988A).