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. 2015 Mar 9;6:186. doi: 10.3389/fmicb.2015.00186

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Copyright © 2015 Martín-Betancor, Rodea-Palomares, Muñoz-Martín, Leganés and Fernández-Piñas.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Scheme of plasmid pBG2120 construction. (A) pBG2120 resulted of cloning a sequence containing the smtB gene, the smt operator/promoter region and the first 50 pb of smtA in SalI/KpnI site of pBG2106. Transcription of the lux operon in pBG2120 is under the control of the smtAB promoter (PsmtAB). (B) Structure of the chromosomal smt locus of Synechococcus sp. PCC 7942. The operator/promoter region lies between the smtA and the smtB protein coding regions and contains divergent overlapping promoters of the two genes [squares indicate the smtA and smtB transcription start site, Shine-Dalgarno sequence (S.D.) and −10 regions are indicated in bold)]. (C) Sequence of PCR primers (shown in bold) used to amplify the smt sequence including smtB, the 100 bp operator/promoter region and 50 bp of smtA.