Table 4.
PUFAs extracted in different microalgae species
| Species | Product | Yield | Extraction/Purification method | Reference |
|---|---|---|---|---|
| Arthrospira platensis | Total lipids | 13.2% DW | Chloroform–methanol 1:1 (%v/v) | Ryckebosch and colleagues (2011) |
| Chlorella vulgaris | 19.9% DW | |||
| Chlorella vulgaris Green cells | ALA | 661 mg/100 g | Acid digestion of biomass with 4 N HCl. | Batista and colleagues (2013) |
| EPA | 19 mg/100 g | |||
| DHA | 16 mg/100 g | Soxhlet method with petroleum ether for 6 h | ||
| GLA | 112 mg/100 g | |||
| Chlorella vulgaris Orange cells | ALA | 3665 mg/100 g | ||
| EPA | 39 mg/100 g | |||
| DHA | 80 mg/100 g | |||
| GLA | 23 mg/100 g | |||
| Crypthecodinium cohnii | DHA | 99.2% purity | Purification by saponification, winterization and urea complexation | Mendes and colleagues (2007) |
| Diacronema vlkianum | ALA | 14 mg/100 g | Acid digestion of biomass with 4 N HCl. | Batista and colleagues (2013) |
| EPA | 3212 mg/100 g | |||
| DHA | 836 mg/100 g | Soxhlet method with petroleum ether for 6 h | ||
| GLA | 112 mg/100 g | |||
| H. pluvialis | ALA | 3981 mg/100 g | ||
| EPA | 579 mg/100 g | |||
| GLA | 472 mg/100 g | |||
| Isochrysis galbana | ALA | 421 mg/100 g | ||
| EPA | 4875 mg/100 g | |||
| DHA | 1156 mg/100 g | |||
| Scenedesmus obliquus | Total Lipids | 29.7% DW | Chloroform–methanol 1:1 (%v/v) | Ryckebosch and colleagues (2011) |
| Spirulina maxima | ALA | 40 mg/100 g | Acid digestion of biomass with 4 N HCl. Soxhlet method with petroleum ether for 6 h | Batista and colleagues (2013) |
| GLA | 452 mg/100 g | |||
| Nannochloropsis salina | Total Lipids | 34.4% DW | Chloroform–methanol 1:1 (%v/v) | Ryckebosch and colleagues (2011) |
| Nannochloropsis gaditana | EPA | 3.7 g/100 g DW | Dichloromethane-ethanol (1:1) | Ryckebosch and colleagues (2013) |
| Parietochloris incise | ARA | 9.1% DW | Methanol (10%DMSO) at 40°C for 5 min. | Bigogno and colleagues (2002) |
| Diethyl ether, hexane and water 1:1:1 (v/v/v) | ||||
| Phaeodactylum tricornutum | EPA | 9.3% DW of total fatty acids (39%) | Chloroform–methanol 1:1 (%v/v) | Ryckebosch and colleagues (2011) |
| Porphyridium cruentum | EPA | 50.8% recovery, 97% purity | Purification by saponification and urea complexation | Guil-Guerrero and colleagues (2000) |
| Tetraselmis sp. | EPA | 10.41% of phospholipid fraction | Chloroform-methanol 2:1 (%v/v). Extract was washed with 0.88% w/v KCl to remove non-lipids | Makri and colleagues (2011) |