Table 2.
Oligonucleotides used in this study
| Name | Sequence (5′→3′) | Restriction sites |
|---|---|---|
| DE3-for | CCGCTCGAGAACTGCGCAACTCGTGAAAGG | XhoI |
| DE3-rev | CGGAATTCGTTACGCGAACGCGAAGTC | EcoRI |
| pETEx-for | AACTGCAGGGAGCTGACTGGGTTGAAGG | PstI |
| pETEx-rev | AACTGCAGCTTAATGCGCCGCTACAGGG | PstI |
| pEKEx-for | GGAATTCCATATGTCGCTCAAGCCTTCGTCACTG | NdeI |
| pEKEx-rev | GGACTAGTTTATCTAGACTTGTACAGCTCGTCCATG | SpeI |
| eYFP-for | CATGCCATGGACAATAACGATCTCTTTCAGGCATCAC | NcoI |
| eYFP-rev | CGGGATCCTCAGCCCGCGAGCACC | BamHI |
| PykCg-for | CGGGATCCGGCGTGGATAGACGAACTAAG | BamHI |
| PykCg-rev | TGTACAGACACCACGTACAGTGTCAACGC | BsrGI |