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. 2015 Jan 23;8(2):266–274. doi: 10.1111/1751-7915.12245

Figure 5.

Figure 5

Determination of DNA content by flow cytometry of S. cerevisiae strains. Strains were grown to exponential phase then fixed with ethanol, and the DNA was stained with propidium iodide. The x-coordinate FL2-A represents total cell fluorescence, and it is proportional to DNA content. Two Gaussian curves in density plot represent the G0/G1 and G2/M phase of cells respectively. The strain ploidy was then determined by the G0/G1 peak value on FL2-A versus that of the haploid strain, which was set as 200.