Table 2.
Production of cadaverine and L-lysine by recombinant B. methanolicus M168-20 strains cultivated at different medium pH
| pH | M168-20(pHP13) | M168-20(pTH1mp-ldcC) | M168-20(pTH1mp-cadA) | |||
|---|---|---|---|---|---|---|
| Cadaverine | L-lysine | Cadaverine | L-lysine | Cadaverine | L-lysine | |
| 6.5 | 0 | 50 ± 10 | 52 ± 5 | < 15 | 45 ± 5 | < 15 |
| 7.2 | 0 | 130 ± 10 | 135 ± 10 | 40 ± 5 | 430 ± 20 | < 30 |
| 7.6 | 0 | 140 ± 10 | 315 ± 20 | < 30 | 450 ± 20 | < 30 |
| 8.0 | 0 | 140 ± 10 | 305 ± 30 | < 15 | 500 ± 30 | < 30 |
| 8.5 | 0 | 140 ± 10 | 305 ± 30 | < 15 | 520 ± 30 | < 30 |
The mean values (mg/L) and standard deviation of triplicate shake flask cultures is presented. The production levels were found from late stationary cultures, from 20–30 h after inoculation.