Table 5.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference |
|---|---|---|
| E. coli | ||
| DH5α | General cloning host | Stratagene |
| MG1655 | Wild type strain | ATCC 47076 |
| B. methanolicus | ||
| MGA3 | Wild-type strain | ATCC 53907 |
| M168-20 | AEC-resistant hom1 MGA3 mutant | (Brautaset et al., 2010) |
| Plasmids | ||
| pHP13 | E. coli-B. subtilis shuttle vector, Clmr | (Haima et al., 1987) |
| pTH1mp-lysC | pHP13 derivate with lysC under control of mdh promoter | (Brautaset et al., 2010) |
| pTH1mp-lysA | pHP13 derivate with lysA under control of mdh promoter | (Nærdal et al., 2011) |
| pTH1mp-ldcC | pHP13 derivate with ldcC under control of mdh promoter | This study |
| pTH1mp-cadA | pHP13 derivate with cadA under control of mdh promoter | This study |
| pTH1mp-ldcC-lysC | pTH1mp-ldcC with lysC downstream of the ldcC gene | This study |
| pTH1mp-ldcC-lysA | pTH1mp-ldcC with lysA downstream of the ldcC gene | This study |
| pTH1mp-cadA-lysA | pTH1mp-cadA with lysA downstream of the cadA gene | This study |
Clmr, chloramphenicol resistance.