Table 2.

Growth and production parameters of strains MV-KICF1 and SH-KIC20 in shake flask cultivations^ab

Parameter	MV-KICF1 + 1 mM l-isoleucine	MV-KICF1 without l-isoleucine	SH-KIC20d without l-isoleucine
Growth rate (h−1)	0.21 ± 0.01	0.03 ± 0.01	0.08 ± 0.01
KIC (mM)	46.7 ± 4.1	31.8 ± 2.1	18.8 ± 0.67
By-productsc:
KIV (mM)	13.3 ± 2.2	19.0 ± 4.1	2.6 ± 0.3
KMV (mM)	8.8 ± 1.3	4.9 ± 0.6	8.7 ± 0.1
l-leucine (mM)	3.0 ± 0.2	10.3 ± 3.1	4.8 ± 0.2
Molar product yield (mol KIC per mol glucose)	0.204 ± 0.018	0.143 ± 0.010	0.084 ± 0.001
Volumetric productivity (mmol KIC l−1 h−1)	1.41 ± 0.13	0.34 ± 0.02	0.38 ± 0.02

^aCultivations were performed in 500 ml baffled shake flasks containing 50 ml CGXII minimal medium with 4% (w/v) glucose. Supplementation of l-isoleucine is indicated.

^bMean values and standard deviations from three independent cultivations are shown.

^cConcentrations of l-valine and l-isoleucine were below 2 mM.

^dCultivation of SH-KIC20 supplemented with 1 mM l-isoleucine was not tested.