Table 3.
Putative transporter genes showing increased expression in a KIC producer
| Gene | Annotation | mRNA ratioa (MV-Leu20 ΔilvE/ Wild type | TMHb |
|---|---|---|---|
| cg0018 | putative membrane protein, conserved | 3.5 | 9 |
| cg1121 | putative permease of the major facilitator superfamily | 2.2 | 7 |
| cg1219 | putative membrane protein | 3.5 | 10 |
| cg1419 | putative Na+-dependent transporter, bile acid:Na+ symporter BASS family | 7.2 | 8 |
| cg1658 | putative permease of the major facilitator superfamily | 35.6 | 12 |
| cg2557 | putative secondary Na+/bile acid symporter, bile acid:Na+ symporter BASS family | 2.5 | 8 |
| cg2676 | putative ABC-type dipeptide/oligopeptide/nickel transport system, permease component | 2.1 | 6 |
| cg3334 | putative arabinose efflux permease, MFS type | 2.0 | 12 |
a
Transcriptome analyses of KIC producer MV-Leu20 ΔilvE in comparison to the wild type were performed using DNA microarrays as described (Vogt et al., 2014). Candidate transporter genes were chosen based on an mRNA ratio (MV-Leu20 ΔilvE/wild type) of > 2, an annotation as (putative) membrane or transporter proteins and the prediction of multiple transmembrane helices in the encoded proteins. Data represent mean values of at least two (maximum four) evaluable microarray experiments (P-value < 0.05).
b
TMH, number of transmembrane helices predicted with the sosui engine version 1.11.