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. 2015 Mar 9;6:174. doi: 10.3389/fmicb.2015.00174

FIGURE 5.

FIGURE 5

(A) SDS-PAGE analysis of the purity of purified recombinant SA1804; (B) Electrophoretic mobility shift analysis of the hla promoter regulated by SA1804. The promoter region of the hla gene was obtained by PCR, purified, and labeled with Digoxigenin. The mobility of the labeled promoter fragment without addition of SA1804 is shown in the first lane. Different amounts of SA1804 protein (25, 100, 250, 500 ng) were incubated with each labeled hla promoter probe in a 15 μl reaction volume. Specific competitor (SC) control: incubation in the presence of 100-fold excess unlabeled SC. Non-specific competitor (NSC) control: incubation in the presence of 100-fold excess unlabeled non-specific internal gene probe. BSA (1 μg) was used as non-specific binding control.