Table II.
The Amino Acids that Define the AA Binding Site in 8R-LOX and Their Counterparts in Other LOX Structures
| 8-R-LOX identity | 11-R-LOX 47% | 5 -LOX 38% | 15-LOX-2 33% | 15-LOX 30% | 12-LOX 30% | LOX1 28% | LOX3 28% | LOXB 27% | LOXD 27% | PA 33% |
|---|---|---|---|---|---|---|---|---|---|---|
| 4QWT | 3FG1 | 3O8Y | 4NRE | 2P0M | 3RDE | 1YGE | 1LNH | 2IUJ | 2IUK | 4G32 |
| Tyr-178 | Phe-185 | Phe-177 | Phe-184 | Phe-175 | Phe-175 | Ala-254 | Phe-272 | Phe-267 | Phe-277 | -- |
| Arg-182 | Gly-188 | Tyr-181 | Ala-188 | Leu-179 | Leu-179 | Gly-258 | Gly-276 | Gly-271 | Gly-281 | -- |
| Gln-380 | Gln-369 | Gln-363 | Glu-369 | Gln-357 | Glu-357 | Gln-495 | Gln-514 | Gln-509 | Gln-521 | Glu-373 |
| His-384 | 373 | 367 | 373 | 361 | 361 | 499 | 518 | 513 | 525 | 377 |
| Leu-385 | 374 | 368 | 374 | 362 | 362 | Trp-500 | Trp-519 | Trp-514 | Trp-526 | 378 |
| His-389 | 378 | 372 | 378 | 366 | 366 | 504 | 523 | 518 | 530 | 382 |
| Ile-423 | 412 | 406 | 412 | 400 | 400 | 538 | 557 | 552 | 564 | I416 |
| Gly-427 | Gly-416 | Ala-410 | Ala-416 | Ala-404 | Ala-404 | Ala-542 | Ala-561 | Ala-556 | Ala-568 | Ala-420 |
| Leu-431 | 420 | 414 | 420 | 408 | 408 | 546 | 565 | 560 | 572 | 424 |
| Ile-437 | Ala-426 | Leu-420 | Val-426 | Ile-414 | Ile-414 | Ile-552 | Val-571 | Ile-566 | Ile-578 | Phe-430 |
| Val-438 | Ala-427 | Phe-421 | Val-427 | Phe-415 | Phe-415 | Ile-553 | Ile-572 | Ile-567 | Ile-579 | Ile-430 |
| Leu-442 | Leu-431 | Asn-425 | Thr-431 | Met-419 | Leu-419 | Phe-557 | Phe-576 | Phe-571 | Phe-583 | Phe-435 |
| Ala-589 | Thr-575 | Pro-569 | Pro-572 | Cys-560 | Cys-560 | Thr-709 | Thr-728 | Thr-723 | Thr-735 | Ala-574 |
| Ala-620 | Met-606 | His-600 | Val-603 | Gln-590 | Gln-590 | Ser-747 | Asp-766 | Asp-761 | Asp-773 | Lys-605 |
| Thr-623 | Val-609 | Ala-603 | Ala-606 | Ile-593 | Ile-593 | Val-750 | Val-769 | Ile-764 | Ile-766 | Ile-608 |
| Ile-626 | Ala-612 | Ala-606 | Leu-609 | Gln-596 | Gln-596 | Ile-753 | Ile-772 | Ile-767 | Ile-779 | Leu-611 |
| Leu-627 | 613 | 607 | 610 | 597 | 597 | 754 | 773 | 768 | 780 | 612 |
Overall sequence identity with P. homomalla 8R-LOX is in indicated. The amino acids of the highly conserved active site core are in italics and shown in Bold font for reference structure.