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HCD type MS2 fragmentation spectra obtained at low [30 NCE, (A)], middle [70 NCE, (B)], and high [110 NCE, (C)] energy for precursor mass 340.1755 m/z, with the proposed elemental formula of C17H25NO6. The key fragment C4H5O2, marked with a dashed oval, and the neutral loss C3H9N, indicated by a dashed arrow, occur in the spectra, and this fragmented metabolite could be annotated as an acylcarnitine.
