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. Author manuscript; available in PMC: 2015 Mar 9.
Published in final edited form as: Arch Biochem Biophys. 2013 Sep 10;539(1):31–37. doi: 10.1016/j.abb.2013.09.001

Figure 3.

Figure 3

Catalytic constants of F226 variants via 18O exchange. Data for each variant are represented throughout as follows: HCA II, solid black square (■); F226I, open triangle (Δ); F226L, cross (X); F226W, open circle (○). (A) Calculation of kcat/KM (M−1 μs1) versus pH from Eq. 3. (B) The pH profile for the rate constant RH2O/[E] (μs−1) used in calculation of the proton transfer constant, kB, and pKa values via Eq. 4.