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. Author manuscript; available in PMC: 2015 Mar 9.
Published in final edited form as: Arch Biochem Biophys. 2013 Sep 10;539(1):31–37. doi: 10.1016/j.abb.2013.09.001

Table 2.

Apparent Values of pKa and Maximal Rate Constants for F226 HCA II Variants.

Variant kcat/KM (M−1 μs−1)a pKaZnOHb pKaHis64b kB (μs−1)b
HCA II 120 ± 4 7.0 ± 0.1 6.8 ± 0.1 1.4 ± 0.2
F226I 118 ± 4 6.8 ± 0.1 6.6 ± 0.1 1.4 ± 0.2
F226L 139 ± 4 6.7 ± 0.2 6.9 ± 0.2 1.3 ± 0.2
F226W 87 ± 3 7.0 ± 0.2 6.9 ± 0.2 1.0 ± 0.3
a

Measured from the data in Fig. 3A using Eq. 3.

b

Measured from the data in Fig. 3B using Eq. 4.