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. Author manuscript; available in PMC: 2015 Mar 9.
Published in final edited form as: Arch Biochem Biophys. 2013 Sep 10;539(1):31–37. doi: 10.1016/j.abb.2013.09.001

Table 3.

Thermodynamic properties of F226 HCA II Variants.

Property HCA II F226I F226L F226W
TM (K)a 330.2 ± 0.1 326.1 ± 0.1 326.3 ± 0.1 324.1± 0.2
327.2± 0.7
329.2 ± 0.2
ΔH°m(kcal mol−1)a 223 ± 5 104 ± 4 208 ± 9 110 ± 9
71 ± 11
25 ± 3
ΔHvH(kcal mol−1)b 195 ± 6 113 ± 10 114 ± 6 140 ± 3
240 ± 20
480 ± 40
ΔCp (kcal mol−1 K−1)c 0.69 0.32 0.65 0.32
0.22
0.67
ΔGT (kcal mol−1)d 1.98 −0.63 −1.21 −1.31
−0.21
0.081
ΔHT (kcal mol−1)e 311 103 212 107
25
71
ΔST (kcal mol−1 K−1)f 0.93 0.32 0.65 0.33
0.67
0.076
a

Calorimetric parameters determined by DSC.

b

van't Hoff enthalpy (ΔHvH) was determined by fitting thermograms to a two-state reversible unfolding model.

c

Heat capacity (ΔCp) of protein unfolding obtained by plotting calorimetric enthalpy (ΔH°m) versus melting temperature (TM).

d

Thermodynamic parameter extrapolated to reference temperature of 328.2 K using Eq. 5.

e

Thermodynamic parameter extrapolated to reference temperature of 328.2 K using Eq. 6.

f

Thermodynamic parameter extrapolated to reference temperature of 328.2 K using Eq. 7.