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. 2014 Nov 6;(93):52021. doi: 10.3791/52021
Yeast Genotype Source
TRy467 α, his3∆1, lys2∆0, ura3∆0, leu2∆0 Euroscarf
TRy508 α, his3-∆200::pRS303/Ubc7prom-Ubc7-3HA::HIS,  leu2-3,112,  ura3-5, lys2-801::Met25-Deg1-Flag-Vma12-Ura3::LYS,  trp1-1, ubc7∆::LEU2 This study
TRy528 α, his3-∆200::pRS303/Ubc7prom-Ubc7[N81A]-3HA::HIS, leu2-3,112,  ura3-5, lys2-801::Met25-Deg1-Flag-Vma12-Ura3::LYS,  trp1-1, ubc7∆::LEU2 This study
TRy530 α, his3-∆200::pRS303/Ubc7prom-Ubc7[C89S]-3HA::HIS, leu2-3,112,  ura3-5, lys2-801::Met25-Deg1-Flag-Vma12-Ura3::LYS,  trp1-1, ubc7∆::LEU2 This study
TRy532 α, his3-∆200::pRS303/Ubc7prom-Ubc7[H94K]-3HA::HIS, leu2-3,112,  ura3-5, lys2-801::Met25-Deg1-Flag-Vma12-Ura3::LYS,  trp1-1, ubc7∆::LEU2 This study
TRy556 α, his3-∆200::pRS303::HIS, leu2-3,112,  ura3-5, lys2-801::Met25-Deg1-Flag-Vma12-Ura3::LYS,  trp1-1, ubc7∆::LEU2 This study
TRy563 α, his3-∆200::pRS303/Ubc7prom-Ubc7-3HA::HIS, leu2-3,112,  ura3-5, lys2-801::Met25-Deg1-Flag-Vma12-Ura3::LYS,  trp1-1, ubc7∆::LEU2, ubc6∆::KanMX  This study
TRy633 α, his3-∆200::pRS303/Ubc7prom-Ubc7[V25A]-3HA::HIS, leu2-3,112,  ura3-5, lys2-801::Met25-Deg1-Flag-Vma12-Ura3::LYS,  trp1-1, ubc7∆::LEU2 This study
TRy786 α, his3-∆200::pRS303/Ubc7prom-Ubc7-3HA::HIS, leu2-3,112,  ura3-5, lys2-801::Met25-Deg1-Flag-Vma12-Ura3::LYS,  trp1-1, ubc7::LEU, doa10∆::HIS3 This study