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. 2014 Nov 6;(93):52021. doi: 10.3791/52021
Plasmids used in this study
Plasmid Relevant markers Source
pTR717 YDpK-MET25p-Deg1-Flag-Vma12-Ura3 (integrative plasmid containing LYS1) This study
pTR1412 pRS315-CUP1p-URA3-HA-GFP (LEU) This study
Additional suitable reporter plasmids
Plasmid Relevant markers Source
pOC9-CL1 pOC9-CUP1p-HA-URA3 (TRP1) (17)
URA3-2(GFP) pPS414-TRP1p-MYC-ura3-2-GFP Lewis and Pelham