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Methylation-specific primers of SNCA and FBN1.
| Primer set | Forward primer | Reverse primer | Amp size (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
| SNCA-M | CGGGTTGTAGCGTAGATTTC | CGTCGAATAACCACTCCC | 125 | 53 |
| SNCA-U | GTGTGGGTTGTAGTGTAGATTTT | TCATCAAATAACCACTCCCAA | 129 | 53 |
| FBN1-M | GTATTTTTTTCGCGAGAAATC | AATCGTAACCGCTACAACC | 164 | 48 |
| FBN1-U | AAAGTATTTTTTTTGTGAGAAATT | CCCAATCATAACCACTACAACC | 170 | 48 |
