Figure 4.

Inhibitory effects of OYSGS extract on the productions of leptin in 3T3-L1 adipocytes. Cell differentiation was induced for 8 days with or without OYSGS. (a) Cell lysates were prepared and used for analyzing the GPDH activity. (b) Leptin concentration in the supernatant was measured using a leptin quantification kit. Results are presented as the mean ± SEM. ^** P < 0.01 and ^*** P < 0.001 compared with the differentiated group. The graph represents the GPDH activity (a) and leptin production (b) from three independent experiments.