Table 1.

Primers and PCR conditions used to amplify resistance genes

Resistance genes	Primers	PCR conditions	References
ermA	F 5′TCT AAA AAG CAT GTA AAA GAA3′	Pre cycle 93°C 3 min,	[7] Sutcliffe 1996
	R 5′CTT CGA TAG TTT ATT AAT ATT AGT3′	35 cycles: 93°C 60 s, 52°C 60 s, 72°C 60 s
ermB	F 5′GAA AAG GTA CTC AAC CAA ATA3′	Last cycle 72°C 5 min
	R 5′AGT AAC GGT ACT TAA ATT GTT TAC3′
ermC	F 5′GCT AAT ATT GTT TAA ATC GTC AAT TCC3′
	R 5′GGA TCA GGA AAA GGA CAT TTT AC3′
msrA	F 5′GCA AAT GGT GTA GGT AAG ACA ACT3′	Pre cycle 93°C 3 min,	[7] Sutcliffe 1996
	R 5′ATC ATG TGA TGT AAA CAA AAT3′	35 cycles: 93°C 30 s, 52°C 30 s, 72°C 60 s
		Last cycles 72°C 10 min
linA	F 5′GTA TTA ACT GGA AAA CAG CAA AG3′	Pre cycle 5 dk 94°C	[10] Lina 1999
	R 5′GAG CTT CTT TTG AAA TAC ATG G3′	35 cycles 45 s 94°C, 45 s 54°C,1 min at 72°C
linB	F 5′CCTACCTATTGTTTGTGGAA 3′	Last cycle 5 min at 72°C	[11] Bozdogan 1999
	R 5′ATAACGTTACTCTCCTATTC 3′
tetM	F 5′GTG GAC AAA GGT ACA ACG AG3′	Pre cycle 93oC’de 5 dk	[9] Warsa 1996
	R 5′CGG TAA AGT TCG TCA CAC AC3′	35 cycles 93°C 60 s, 52°C 60 s, 72°C 60 s
tetK	F 5′CAG CAG ATC CTA CTC CTT3′	Last cycle 10 min at 72°C
	R 5′TCG ATA GGA ACA GCA GTA3′
aac-aph	F 5′GAG CAA TAA GGG CAT ACC AAA AAT C3′	Pre cycle 94C’de 5 dk,	[8] Kao 2000
	R 5′CCG TGC ATT TGT CTT AAA AAA CTG G3′	35 cycles 94°C 30 s, 50°C 30 s, 72°C 30 s
		Last cycle 7 min at 72°C