Table 3.

Primers used in MiIFN-α and mature peptide (mMiIFN-α) gene PCR assays

Gene fragment	Predicted size (bp)	Primers	Tm( ° C)
MiIFN-α1–13	564	P1 5′-ATGGCCCTGCCCTGCTCCT- 3′	50.1
		P2 5′-TCACTTCCTGCTCCGCAATC-3′
mMiIFN-α1–13	495	P3 5′- CGGGATCCTGTGACCTGCCTCAG-3′	55
		P4 5′- CCAAGCTTTCACTTCCTGCTCCGCAAT-3′