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. Author manuscript; available in PMC: 2015 Mar 9.
Published in final edited form as: Cancer Gene Ther. 2014 May 30;21(6):228–237. doi: 10.1038/cgt.2014.22

Figure 2. Cytolytic activity of E1B mutants in hamster cell lines.

Figure 2

(A). Expression of cytokeratins in hamster cell lines. The western blots were probed with antibodies against human cytokeratins 8, 14 and 19, EGFR and actin. (B). Cytolytic activity of HAdV5 mutants. The hamster cell lines HPT11 and 12 were infected with HAdV5 wt or mutants at 500 PFU/cell and the cytolytic activity was determined by the lactate dehydrogenase (LDH) release assay using CytoTox 96 Non-Radioactive cytotoxicity assay kit (Promega).