Table 3.

HMB and metabolites concentrations (μg/mL) in serum of dams on GD 10, GD 15, GD 20 and PND 23

	Control		HMB dose group (ppm)
	0 ppm		1,000		3,000		10,000		25,000		50,000
	HMB	DHB	HMB	DHB	HMB	DHB	HMB	DHB	HMB	DHB	HMB	DHB
GD 10	<LOD	<LOD	<LOD	0.0422 ± 0.0138	0.0174 ± 0.0062	0.2406 ± 0.3618	0.0312 ± 0.0126	0.1652 ± 0.0459	0.1250 ± 0.0294	0.2903 ± 0.0830	0.1886 ± 0.1019	0.3097 ± 0.1118
N	6		6		6		6		6		6
GD 15	<LOD	<LOD	<LOD	0.0425 ± 0.0137	0.0062 ± 0.001	0.0571 ± 0.0171	0.0481 ± 0.0151	0.2155 ± 0.0392	0.1385 ± 0.0161	0.3070 ± 0.0359	0.2318 ± 0.1070	0.4012 ± 0.1401
N	6		6		6		6		6		6
GD 20	<LOD	<LOD	<LOD	0.0307 ± 0.0187	0.0096 ± 0.0009	0.0812 ± 0.0314	0.0660 ± 0.0417	0.1774 ± 0.0862	0.1466 ± 0.0721	0.3147 ± 0.1866	0.4850 ± 0.2496	0.6215 ± 0.2762
N	6		5		6		6		6		6
PND 23	0.0118 ± 0.0070	<LOD	0.0072 ± 0.0008	0.0382 ± 0.0122	0.0164 ± 0.0139	0.1284 ± 0.0423	0.1011 ± 0.0310	0.2562 ± 0.0599	0.4804 ± 0.0899	0.7748 ± 0.1397	0.6886 ± 0.2447	1.0066 ± 0.3874
N	5		5		5		5		5		5

Values are mean ± SD. N indicates number of litter per dose group. LOD of HMB, DHB and DHMB is < 0.005 μg/ml. LOD of THB is < 0.1 μg/ml (GD 10, 15 and PND 23) or 0.05 μg/ml (GD 20). THB and DHMB concentrations were under the limit of detection (LOD) in all analyses.