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. 2015 Mar 9;10(3):e0118659. doi: 10.1371/journal.pone.0118659

Fig 5. Effects of pontin depletion on the expression of E-cadherin and vimentin, and β-catenin subcellular localization in vitro.

Fig 5

(A) Effects on the protein expression of pontin, E-cadherin and vimentin after siRNA transfection for 72 h detected by western blotting. The data shown is representative of 3 independent experiments. (B) The subcellular location of β-catenin was measured by fluorescent immunocytochemical analysis after siRNA transfection for 72 h (green, β-catenin; blue, DAPI nuclear staining). Magnification: ×600. (C) Time dependent changes in mRNA levels of pontin, c-myc and cyclin D1 after siRNA transfection for 48h, 72h and 96h detected by qRT-PCT. Data were shown as mean ± s.e.m. (n = 3). β-actin was used as a loading control. *P<0.05, **P<0.01, ***P<0.001.