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. 2015 Mar 9;10(3):e0118792. doi: 10.1371/journal.pone.0118792

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© 2015 Cramer et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — (A) Experimental design of three-dimensional assay. Expanded intestinal cells were embedded as single cells in Matrigel, first expanded in Stem Cell (SC) media, then treated further with SC media, Differentiation media or Differentiation media + DAPT (GSI). (B) In vitro and H&E pictures of SI (top) and LI (bottom) organoids in each of the three treatments. One paired set of SI and LI organoids is representative of three sets examined. Scale bar = 50μm.