Skip to main content
. Author manuscript; available in PMC: 2015 Mar 9.
Published in final edited form as: Chem Biol. 2008 Apr;15(4):322–331. doi: 10.1016/j.chembiol.2008.03.005

Figure 6. IPA-3 selectively inhibits Pak1 activation in mammalian cells.

Figure 6

Mouse embryonic fibroblasts were serum starved for 18 hours, followed by treatment with 30 µM IPA-3, PIR-3.5 (right panels) or DMSO for 10 min. Cells were stimulated with 10 µg/ml PDGF-BB for 5 min and lysed. Cell lysates were normalized by protein concentration and probed with antibodies as described in Materials and Methods. Endogenous Pak kinase activity was observed by in-gel kinase assay.