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. Author manuscript; available in PMC: 2015 Mar 9.
Published in final edited form as: Neuron. 2011 Feb 10;69(3):523–535. doi: 10.1016/j.neuron.2010.12.036

Figure 4. Co-localization of Syt4 with OXT in hypothalamic PVN.

Figure 4

A. OXT (upper panels, green) or AVP (lower panels, green) in the PVN was co-immunostained with Syt4 (all panels, red) and merged to display their co-localization (indicated by yellow color). Scale bar=50 μm.

B. High-magnification images of Syt4 (red) and OXT (green) co-immunostaining. Yellow color in merged images indicates intracellular co-localization of Syt4 and OXT. DAPI staining (blue) revealed nuclei of all cells in the sections. Scale bar=5 μm.

C. OXT and Syt4 co-immunogold labeling in OXT vs. AVP axonal terminals. The posterior pituitary from normal C57BL/6 mice were sectioned and co-immunogold labeled with OXT (small particles) and Syt4 (large particles). The image represents a junction region that contains both OXT axonal terminals and AVP axonal terminals (separated by a blue dotted line). Red arrows indicate dense-core vesicles, and green arrows indicate micro-vesicles. Scale bar=100 nm.

A–C: All experimental mice were adult males, chow-fed, and in C57BL/6 background.