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. Author manuscript; available in PMC: 2016 Feb 2.
Published in final edited form as: Curr Protoc Stem Cell Biol. 2015 Feb 2;32:1H.8.1–1H.8.20. doi: 10.1002/9780470151808.sc01h08s32

Figure 2. Characterization of undifferentiated hPSCs.

Figure 2

hPSCs displayed a typical undifferentiated morphology, including tightly packed colonies of cells and clearly defined edges (A). RT-PCR analysis demonstrated the expression of characteristic pluripotency makers in hPSCs, while lacking mesodermal, endodermal and ectodermal markers (B). Immunocytochemistry further demonstrated widespread expression of pluripotency-associated transcription factors (C–E) as well as cell surface markers (F–H). See Tables 1 and 2 for a listing of primers and antibodies used for RT-PCR and immunocytochemistry.