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. Author manuscript; available in PMC: 2016 Feb 2.
Published in final edited form as: Curr Protoc Stem Cell Biol. 2015 Feb 2;32:1H.8.1–1H.8.20. doi: 10.1002/9780470151808.sc01h08s32

Figure 3. Induction of hPSCs to a Neural Progenitor Fate.

Figure 3

After ten days of differentiation and plating (A), differentiating hPSCs were analyzed by RT-PCR and demonstrated expression of the neural markers PAX6 and SOX1. An anterior neural, eye-field fate was further indicated by the expression of OTX2. RAX, SIX3, and LHX2 (B). Immunocytochemistry demonstrated the widespread expression of many of these transcription factors (C–E).