Table 2.
Primers used for RT-PCR and quantitative RT-PCR.
| Gene amplified | Forward | Reverse | Size (bp) |
|---|---|---|---|
| α-Fetoprotein | AGA ACC TGT CAC AAG CTG TG | GAC AGC AAG CTG AGG ATG TC | 676 |
| ARRESTIN | ACA AGC TAG GGG ACA ATG CC | TTG TGC TAG AGG CCA GGT TG | 597 |
| BEST1 | GGT GTG GTT TGC CAA CCT GTC AAT | TGT TCA TCT CGT TCA GCA GGC TCT | 92 |
| BRACHYURY | ACC CAG TTC ATA GCG GTG AC | CAA TTG TCA TGG GAT TGC AG | 218 |
| BRN3 | CTC ACA CTG TCC CAC AAT AAT A | CCG GCG GAA TAT TTC ATT CT | 311 |
| CHX10 | ATT CAA CGA AGC CCA CTA CCC AGA | ATC CTT GGC TGA CTT GAG GAT GGA | 229 |
| CRX | TAT TCT GTC AAC GCC TTG GCC CTA | TGC ATT TAG CCC TCC GGT TCT TGA | 253 |
| DLX1 | CAA CCA GCA AAT GTC TCC TTC TC | CGC ACT TCA CCG CCT TCC | 282 |
| EMX1 | AGA CGC AGG TGA AGG TGT GG | CAG GCA GGC AGG CTC TCC | 403 |
| EZRIN | ACC ACC ATG GAT GCA GAG CTG GA | ACA CTT CCC GGA GGC CGA TAG T | 100 |
| FABP7 | AGG CAG GTG GGA AAT GTG AC | CAT AGT GGC GAA CAG CAA CC | 298 |
| ISLET1 | GTG TGA TCC GGG TCT GGT TT | AAT TAG AGC CCG GTC CTC CT | 300 |
| KLF4 | AGT CCC GCC GCT CCA TTA CCA A | TGC TCG GTC GCA TTT TTG GCA C | 316 |
| LHX2 | CAA GAT CTC GGA CCG CTA CT | CCG TGG TCA GCA TCT TGT TA | 284 |
| LIN28 | AGT GGT TCA ACG TGC GCA TGG G | AGG TCC GGT GAC ACG GAT GGA T | 203 |
| NANOG | CAA AGG CAA ACA ACC CAC TT | TCT GCT GGA GGC TGA GGT AT | 158 |
| NEUROD1 | TAC TGC TGC AAA GTG CAA ATA C | AAG TGC TAA GGC AAC ACA ATA AC | 539 |
| NEUROD4 | AGG TCT GGG CTC CCA AAA TG | GCC CCG GAG ACT GAT AGT TG | 557 |
| OCT4 | CGA GCA ATT TGC CAA GCT CCT GAA | TTC GGG CAC TGC AGG AAC AAA TTC | 324 |
| OPSIN | GAA GTT CAA GAA GCT GCG CC | TCT CAC ATT GCC AAA GGG CT | 253 |
| OTX2 | CAA CAG CAG AAT GGA GGT CA | CTG GGT GGA AAG AGA GAA GC TG | 429 |
| PAX6 | CGG AGT GAA TCA GCT CGG TG | CCG CTT ATA CTG GGC TAT TTT GC | 300 (+5a) 258 (−5a) |
| PEDF | AGA TCT CAG CTG CAA GAT TGC CCA | ATG AAT GAA CTC GGA GGT GAG GCT | 127 |
| RAX | GAA TCT CGA AAT CTC AGC CC | CTT CAC TAA TTT GCT CAG GAC | 279 |
| RPE65 | TAC CAC AGA AGG TTC ATC CGC ACT | GGG AAA GCA CAG GTG CCA AAT TCT | 92 |
| SIX3 | CGA GCA GAA GAC GCA TTG CTT CAA | CGG CCT TGG CTA TCA TAC ATC ACA | 394 |
| SIX6 | ATT TGG GAC GGC GAA CAG AAG ACA | ATC CTG GAT GGG CAA CTC AGA TGT | 385 |
| SOX1 | CAA TGC GGG GAG GAG AAG TC | CTC TGG ACC AAA CTG TGG CG | 464 |
| SOX2 | CCC CCG GCG GCA ATA GCA | TCG GCG CCG GGG AGA TAC AT | 448 |
| TRANSDUCIN | CAC GAT GCC CAA GGA GAT GT | GGT GGT TGC AGA TGC TGT TG | 419 |