Figure 5.
Two-subunit Yps1 is enriched in the secreted shed species compared to the cell-associated forms. Yeast yps1Δ cells transformed with plasmid Yps1 was grown 24 h at the indicated pH and aliquots from the cell extract (C; 5 × 106 cells equivalent) and concentrated supernatant (S; 10-times more material compared to cell extract) were treated with Endo Hf, separated by 10% SDS-PAGE and immunodetected with an Yps1 antiserum recognizing both the β and α subunits (294–3). The ratio of the immunoreactive Yps1 α subunit over the total Yps1 immunoreactive material (α/T) is shown at the bottom of each lane.