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O -glycosylation of the Yps1 S/T-rich domain involves the Pmt2 O -mannosyltransferase. Yeast simple and double mutants transformed with Yps1 were grown 24 h at pH 3.0 and equal amounts of cell extracts treated with Endo Hf were analyzed by 10% SDS-PAGE and immunoblotted with an Yps1 antiserum recognizing both the β and α subunits (294–3).
