Skip to main content
. 2015 Feb 26;15:50. doi: 10.1186/s12866-015-0380-1

Table 1.

N-terminal sequencing of purified Yps1 species

Sequenced sample Culture medium pH Molecular mass (kDa) Identified residues at each cycle* Deduced sequence
yps1∆ Yps1-DL 3.0 48 A1D2G3Y4E5E6I7I8I9T10N11Q12Q13S14F15 A68DGYEEIIITNQQSF
yps1∆ ssYps1-DL 6.0 48 A1D2G3Y4E5E6I7I8I9T10N11Q12Q13S14F15 A68DGYEEIIITNQQSF
yps1∆ ssYps1-DL 6.0 52 D1D2D3S4N5S6K7F8V9K10L11P12F13H14(K)15 D31DDSNSKFVKLPFHK
yps1∆kex2∆ ssYps1-DL 6.0 54 K1I2I3P4A5A6N7K8R9D10D11D12S13N14S15 K22IIPAANKRDDDSNS

*:number in exponent matches the cycle number.