Table 4.
Plasmids used in this study
| Plasmid | Description | Source |
|---|---|---|
| pRS426 | 2 μ replication origin, URA3 marker | [34] |
| pYPS1 | pRS426 expressing the YPS1 gene from its own promoter | [8] |
| pYPS1-C2 | pYPS1 with D101E mutation | [8] |
| pYPS1-DL | pYPS1 in which the Yps1p N-ent loop is replaced by the Yps3p codons 98 to 102 | This study |
| pYPS1-DL2 | pYPS1-DL with D101E mutation | This study |
| pBA22H (pssYPS1) | 2 μ replication origin, expressing a truncated version of Yps1p (Δ67 C-ter) with a 6×His-tag | [33] |
| pssYPS1-DL | pssYPS1 in which the Yps1 N-ent loop is replaced by the Yps3p codons 98 to 102 | This study |
| pssYPS1-DL2 | pssYPS1-DL with D101E mutation | This study |